Effect of Bacillus spp. on Lettuce Growth and Root Associated Bacterial Community in a Small-Scale Aquaponics System

The integration of probiotics in aquaponics systems is a strategy for mitigating environmental impacts and promoting sustainable agriculture. In order to understand the role probiotics, we investigated effect commercial probiotic mixture Bacillus subtilis B. licheniformis on growth lettuce (Lactuca sativa L.) under deep-water culture integrated with Mozambique tilapia (Oreochromis mossambicus). We determined plant growth, water quality parameters, leaf mineral analysis, assessed influence microbiota. Bacterial communities were analyzed by high-throughput 16S rRNA gene sequencing. Compared control systems, addition significantly increased concentration nitrate phosphate deep solution, which contributed improved growth. both trials, Fv/Fm, mean shoot dry weight, fresh weight harvested shoots from treatment higher than those observed plants. Higher concentrations phosphorus, potassium, zinc leaves found that received Bacillus. Although differences at phylum level, Proteobacteria Bacteroidetes predominant Bacillus-treatment systems. At genus however, present two types heterogeneous Bacillus-treated containing numbers Chryseobacterium, Bacillus, Nitrospira, Polynucleobacter, Thermomonas. results indicate supplementation can effectively alleviate nutrient deficiencies, improve quality, modify composition bacterial